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KMID : 0350519940470041837
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Journal of Catholic Medical College
1994 Volume.47 No. 4 p.1837 ~ p.1849
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Gene Expression of the Cytokines in Mouse Spleen Cell by lonizing Radiation
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Abstract
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Exposure of mammals to ionizing radiation causes the development of a complex, dose-dependent series of potentially fatal physiologic and morphologic changes. Radiation-induced destruction of the lymphoid and hematopoietic systems is the primary
cause
of septicemia and death. It has been suggested that cytokines produced by various stimuli can enhance hematopoietic and immune functions. We performed this study to assess gene expression of cytokine that can be induced in the mouse spleen cell
by
various dosage of ionizing radiation according to the time incubation after irradiation.
Spleen cells from specific pathogen-free BALB/C mice were incubated for 4hr and then irradiated with dosage of 1,000, 2,000 and 3,000cGy, using 6MV X-ray with 100cGy/min dose rate. Irradiated cells were brought back for further incubation for
various
lengths of time. The c DNA was synthesized from the cells immediately obtained and incubated during 30 min, 1hr, 2hr, 3 hr, and 4 hr after irradiation. Using the reverse transcriptation-polymerase chain reaction, we examined the gene expression
of
interleukin(IL)-1β, IL-6 and transforming growth factor(TGF)-β.
@ES The results were as follows:
@EN 1) In 1,000 cGy irradiated cells, expression of the IL-1β gene expression was induced at 30min after irradiation and maintained for 30 min thereafter. TGF-βgene expression was detected immediately after irradiation and observed for 2 hr.
2) In 2,000 cGy irradiated cells, IL-1βgene was expressed immediately after irradiation and prolonged for 1 hr. TGF-βgene expression was detectable immediately after irradiation and prolonged for 3hr.
3) In 3,000 cGy irradiated cells, expression of IL-1βgene was induced immediately after irradiation and was detectable for 3hr. TGF-βgene was expressed immediately after irradiation and was prolonged for 3hr.
4) IL-6 gene was not expressed in all irradiated groups.
We conclude that ionizing radiation can induce the gene expression of IL-1βand TGF-βand that the time and duration of the gene expression are dependent on the dosage of radiation in IL-1βbut less likely in TGF-β. The results of this study
suggest
that splenic irradiation induced-cytokines may enhance the engraftment of donor marrow hematopoietic stem cells in bone marrow transplantation conditioned with supralethal total body irradiation.
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